Fig. 5.

Increased oxidative metabolism and cellular ATP content upon PASK silencing in L6 cells. (A) PASK mRNA levels in L6 clones expressing either scrambled or PASK shRNA in the absence of doxycycline. Data represent the mean ± SD of triplicates per group. (B) Measurement of ¹⁴CO₂ released from indicated L6 clones incubated in [¹⁴C]glucose (n = 3). (C) Measurement of ¹⁴CO₂ released from indicated L6 clones incubated in [¹⁴C]palmitate (n = 3). (D) Cellular ATP content measurement normalized to protein in extract.