Binding of nuclear proteins to the TN promoter Krox element. (A) NIH 3T3, C6, and N2A extracts formed three complexes (complexes 1–3), which are indicated by arrows. (B) Complexes 1 and 2 contain Krox24 and are supershifted (see band labeled supershift) with antibodies to either the N-terminal myc tag or Krox24. Abbreviations for binding components: CD, competitor DNA (μg); NE, nucleus extract (μg); XS, molar excess.