Table 1.

Strains used in this study

Strain	Parental strain	Genotype	Reference
AX4	AX3-K	axeA1,*axeB1,*axeC1* (axeA1, B1, C1)	55
TL72 (csbA)	HL330	axeA1, B1, C1, pyr5-6−† [pΔPYR-BglII], ura+, csbA−‡, csbB−‡, csbC−‡ [csb-KO]	39
TL177 (acbA)	AX4	axeA1, B1, C1, acbA− [acbA-bsr], bsR§	40
DH1	AX3	axeA1, B1, C1, pyr5-6− [pRG24], ura−	56
smlA	DH1	axeA1, B1, C1, pyr5-6− [pRG24], ura+, smlA−¶ [pSmlA-KO]	57
atg8	DH1	axeA1, B1, C1, pyr5-6− [pRG24], ura−, atg8−‖ [atg8-KO], bsR	41
SA550	AX4	axeA1, B1, C1, sglA−** [sglA-KO], bsR	13
SA604	AX3-ORF	axeA1, B1, C1, sgkAOE†† [sgkA-actin 15], G418R‡‡	14

*Mutations required for axenic growth.

^†Uridine synthase.

^‡Contact site B.

^§Blasticidin S resistance.

^¶Small A, aggregate size.

^‖Autophagy regulation.

**S-1-P lyase.

^††Sphingosine kinase A.

^‡‡G418 resistance.