Fig. 1.

Skin graft acceptance in the absence of γ_c despite normal infiltration of activated CD4⁺ T cells. (A) Male skin from alymphoid (Rag2^−/−γ_c^−/−) mice was grafted on female Ml γ_c⁺ mice (Left), Ml γ_c⁻ mice (Center), and Ml γ_c⁻ Bcl-2⁺ mice (Right). Clinical outcome and aspects of the grafts at day 10 and day 120 are shown. Representative examples are shown (n = 10 for Ml γ_c⁺; n = 4 for Ml γ_c⁻; n = 9 for Ml γ_c⁻ Bcl-2⁺). (B) Equal numbers of mature CD4⁺ thymocytes from Ml γ_c⁺ Bcl-2⁺ or Ml γ_c⁻ Bcl-2⁺ mice were adoptively transferred to female Rag2^−/−γ_c^−/− recipients. Male skin was grafted 1 day later. Histological analysis using anti-CD3 antibody at day 10 after transplant is shown. One representative experiment of three is shown. (C) FACS analysis of skin-infiltrating T cells using a combination of CD4, Vβ6, CD44, and CD62L antibodies. CD62L and CD44 expression is shown on gated CD4⁺Vβ6⁺ T cells. One representative experiment of 10 is shown.