Fig. 2.

Normal inflammatory cell recruitment by γ_c⁻ Ml CD4⁺ T cells. (A) Immunohistology of day 10 postgraft skin biopsies using an anti-F4/80 antibody. Analysis was performed on control skin (Left), “surgical skin” (male skin grafted to a control Rag2^−/− recipient; Center Left), male skin graft on Rag2^−/− recipient receiving Ml γ_c⁺ Bcl-²⁺ T cells (Center Right), and male skin graft on Rag2^−/− recipient receiving Ml γ_c⁻ Bcl-2⁺ T cells (Right). One of two independent experiments is shown. (B) FACS analysis of day 10 postgraft skin-infiltrating cells demonstrating presence of macrophages (CD11b⁺Gr1^lo) and neutrophils (CD11b⁺Gr1^hi). No significant difference in the percentage of infiltrating neutrophils (WT, 23 ± 12.6% versus γ_c⁻, 18.2 ± 7.5%; P = NS) was observed (n = 9 for each genotype). (C) FACS analysis showing the infiltration of CD3⁻NK1.1⁺ NK cells. B and C are representative of three independent experiments.