Fig. 3.

CD4⁺ T cells that differentiate in the absence of γ_c are spurious effectors. Ml γ_c⁺ Bcl-2⁺ or Ml γ_c⁻ Bcl-2⁺ thymocytes were adoptively transferred to female Rag2^−/−γ_c^−/− recipients, and male skin grafts were placed 1 day later. (A) Comparison of CD44 and CD62L profiles in spleen and skin Ml γ_c⁺ Bcl-2⁺ T cells at day 10 after graft. Activated effector (CD44^hiCD62L^lo) cells were gated as indicated. (B) Phenotype of spleen (dotted lines) and skin-infiltrating (solid lines) Ml γ_c⁺ Bcl-2⁺ effector CD4⁺ T cells. Mean fluorescence intensity is indicated. (C) γ_c expression conditions the phenotype of skin-infiltrating CD4⁺ T cells. Expression of indicated markers on electronically gated γ_c⁺ Bcl-2⁺ (black lines) and γ_c⁻ Bcl-2⁺ (shaded) CD4⁺Vβ6⁺ T cells is shown. Results are representative of three to six independent experiments. (D) Effector functions in skin-infiltrating CD4⁺ T cells were analyzed at day 10 after graft. Granzyme B (grzB) versus CD62L expression on gated CD4⁺ T cells is shown. (E) IFN-γ production from CD4⁺ T cells was analyzed after overnight restimulation of skin-infiltrating T cells with male APC. Results are representative of six independent experiments.