Fig. 4.

Signaling through CD122 (IL-2Rβ) is required for Ml CD4⁺ T cell differentiation. (A) Absolute numbers of CD4⁺ T cells in thymus and spleen of WT and CD122-deficient Ml female mice. (B) Male skin graft survival is prolonged in the absence of CD122. Male skin grafts were placed on female mice of the indicated genotype (n = 5), and graft survival was monitored. (C) At 10 days after transplant, skin-infiltrating cells were isolated and analyzed by FACS. CD44, CD62L, CD27, Ly6C, and NKG2D expression are shown on electronically gated γ_c⁺ (black lines) and CD122^−/− (shaded histograms) CD4⁺Vβ6⁺ T cells. (D) FACS analysis of granzyme B (grzB) versus CD62L expression on gated CD4⁺ T cells is shown. (E) IFN-γ production from CD4⁺ T cells was analyzed after overnight restimulation of skin-infiltrating T cells with male APC. Results are representative of three independent experiments.