Table 2.
fMet-Phe dipeptide formation with cognate and near cognate codon–anticodon pairs
| Codons | Native (GAA anticodon) |
WT (GAA anticodon) |
G34A (AAA anticodon) |
|||
|---|---|---|---|---|---|---|
| kapp, s−1 | Final fMet-Phe, nM | kapp, s−1 | Final fMet-Phe, nM | kapp, s−1 | Final fMet-Phe, nM | |
| UUU | 1.8 ± 0.4 | 19 ± 4 | 0.8 ± 0.03 | 19 ± 3 | 0.8 ± 0.2 | 17 ± 1 |
| UUC | 1.5 ± 0.2 | 21 ± 8 | 1.0 ± 0.3 | 20 ± 5 | 0.7 ± 0.4 | 20 ± 2 |
| CUU | 0.05 ± 0.02 | 2.5 ± 0.5 | 0.01 ± 0.01 | 19 ± 6 | ND* | ND* |
The data set using native tRNAPhe was determined in ref. 69. ND, not determined.
*Values are too low to be determined.