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. 2007 Sep 14;104(39):15282–15287. doi: 10.1073/pnas.0704089104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 4. — HDAC inhibitors induce p21 and pten gene expression and inhibit neural stem cell proliferation. (a) Gene expression regulated by HDAC inhibitors, TSA, and VPA. “C” indicates solvent control in all sections. (b) VPA treatment led to increased acetylated histone H4 (AcH4) levels in neural stem cells. (c) Increased expression of pten in TLX^−/− adult brains analyzed by RT-PCR analysis. (d) TLX, HDAC3, and HDAC5 were detected on p21 promoter by ChIP assays. (e) Increased expression of pten in TSA- and VPA-treated neural stem cells analyzed by RT-PCR analysis. (f) Cell proliferation as revealed by BrdU labeling (green) in solvent (C) or VPA-treated neural stem cells that were transfected with control GFP siRNA (Upper) or TLX siRNA (Lower). (g) Percent of BrdU⁺ cells in control (bar 1), VPA- (bar 2), TLX siRNA- (bar 3), and TLX siRNA+VPA- (bar 4) treated neural stem cells. Error bars are standard deviation of the mean; assays were repeated three times.