Figure 2.

Effect of flavonoids on U46619- and thrombin-induced [Ca²⁺]_i mobilization. Oregon green BAPTA-1 AM-loaded platelets, or aspirin-treated platelets, were incubated with dimethylsulphoxide (DMSO), flavonoids (50 µm) or with the TP antagonist SQ29548 (1 µm) and stimulated with 2 µm U46619 or 0.5 U ml⁻¹ thrombin. Data represent mean percentage of inhibition compared with mean peak calcium concentration with DMSO [n = 3; *indicates significant differences (P< 0.05) in [Ca²⁺]_i mobilization upon activation with U46619 or thrombin]. Open bars represent U46619-activated platelets; black bars indicate thrombin-stimulation of aspirin-treated platelets. API, Apigenin; GEN, genistein; LUT, luteolin; QUE, quercetin; RUT, rutin; SQ, SQ29548