Table 1.
Dose-dependent inhibition of U46619-induced [Ca2+]i mobilization by flavonoids and by SQ29548
| Flavonoid | IC50 (µmol l−1) |
|---|---|
| Apigenin | 16.6 ± 6.1 |
| Genistein | 15.0 ± 6.1 |
| Luteolin | 10.5 ± 3.4 |
| Quercetin | 26.4 ± 2.2 |
| Rutin | >500 |
| SQ29548 | 0.007 ± 0.003 |
Oregon green BAPTA-1 AM-loaded platelets (200 ×109 l−1) were incubated with increasing concentrations of flavonoids or SQ29548, before U46619 (2 µm) induced [ Ca2+]i mobilization. Dose-dependent inhibition curves were analysed with Ultrafit software to calculate the concentration of flavonoid necessary to obtain half-maximal inhibition of [ Ca2+]i mobilization (IC50). Results are mean ± SD values from three experiments with different platelets.