Figure 3. Protective effect of glutamate receptors antagonists.
Kynurenic acid (1–1.5 mm), a broad-spectrum glutamate receptor antagonist, CNQX (50 μm, AMPA/kainate receptor antagonist) and the selective AMPA receptor inhibitors GYKI52466 (100 μm) and SYM2206 (30 μm) afforded highly significant protection against 60 min OGD. Blocking glutamate release mediated by reverse Na+-dependent glutamate transport with dihydrokainate (DHK, 1 mm) or TBOA (500 μm) was also highly protective. A, representative CAP tracings. B, bar graph showing quantitative changes in mean CAP area recovery after 60 min OGD under different experimental conditions. *P < 10−4versus aCSF alone. n = 23, 8, 6, 6, 5, 3, 6, 5 respectively, as shown in bar graph.