Table 1.

Characterization of immunoisolated neurons

		Fraction of non-neuronal cells (%)
Region	Cells per animal (/1000)	Astroc./Oligodend.	Fibroblast/Microglia	Viability 3 h (%)
Hippocampus	23 ± 8 (36)	0.09 ± 0.18 (4; 2451)	0 (3; 1363)	74 ± 11 (n = 3)
Cerebellum	1715 ± 763 (13)	0.85 ± 0.67 (3; 1726)	0.05 ± 0.08 (1332)	77 ± 12 (n = 3)
RGCs	27 ± 9 (27)	1.18 ± 0.8 (8; 1691)		75 ± 12 (n = 3)

Yield, fraction of non-neuronal cells and viability were determined by haemocytometer counts of tryptan blue-excluding cells, by immunocytochemical staining with cell-type-specific markers and by a microfluorometric survival assay, respectively. Values indicate mean ± s.d. with number of preparations or cells in parentheses.