Cells were rinsed and maintained in HBSS for 2 h containing (A and D) growth media (GM), (B and E) vehicle (dH2O), or (C and F) 2mM AICAR. Cells immunostained for (A, B, C) Oregon Green labeled phalloidin toxin to visualize stress fibers or (D, E, F) monoclonal antibody against vinculin to visualize focal adhesions. Accompanying stellation, (C, arrows) stress fibers are disassembled and (F) focal adhesions are dispersed. Scale bar 25μm. Photomicrographs are representative of 3 independent experiments.