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. Author manuscript; available in PMC: 2008 Sep 7.
Published in final edited form as: Brain Res. 2007 Jul 27;1168:1–10. doi: 10.1016/j.brainres.2007.06.087

Figure 7. AICAR-induced stellation requires AMPK activity.

Figure 7

(A) Compound C prevents AICAR-induced stellation. Cells were infected with GFP adenovirus for 48 h. Then cells were rinsed and maintained in HBSS containing vehicle (DMSO) or 40μM compound C (CC) for 30 min followed by 2 h incubation with 4mM AICAR. Percent stellation was calculated as number of stellate GFP-positive cells divided by total number of GFP-positive cells. Data are mean ± SEM (bars) of 3 independent experiments. * p = 0.0216 (B) Compound C prevents AICAR-induced ACC phosphorylation. Cells were rinsed and maintained in HBSS containing DMSO or 40μM compound C for 30 min followed by 2 h incubation with 2mM AICAR. Western blot reveals diminished ACC phosphorylation in cells treated with compound C. As expected, AMPK phosphorylation is not decreased by CC since this inhibitor decreases AMPK activity but not its phosphorylation by its upstream kinase LKB. The western blot is representative of 3 independent experiments.