Fig. 5.
Effect of exogenous abscisic acid (ABA) treatment on mRNA abundance of cAPX and pAPX and 18S rRNA in Vigna unguiculata ‘EPACE-1’ and ‘1183’ leaves. Exogenous ABA (0.1 mm) was applied by immersing petioles of excised leaves for 30 min (A1), 2 h (A2) and 24 h (A3) at 24 °C and 250 μmol s−1 m−2 light intensity. (A) Gel analysis of APX isoenzyme transcripts. After RT–PCR carried out on 50 ng of total RNA with 30 cycles, samples were visualized using a UV light transilluminator. (B) Relative mRNA levels. The mRNA level of each sample was quantified with the Imager 1D/2D software and normalized to the respective 18S ribosomal RNA. The values represent the mean ± s.d. of three experiments.