Figure 4. Cellular mechanism of αLAG-3 is independent of CD4⁺ cells but dependent on CD8⁺ in ProHA × TRAMP prostate tissue.

Intact or GK1.5-treated ProHA × TRAMP mice were injected with VV-HA or VV-HA/αLAG-3 as described and injected with 10⁶ (A and B) clone 4 CD8⁺ or (C) LAG-3^–/– clone 4 CD8⁺ cells i.v. Mice treated with GK1.5 were bled 1 day before transfer to confirm CD4 depletion. Seven days after cells were transferred, prostates were harvested and single-cell suspensions were prepared as described. Flow cytometry was performed on cells using CD8⁺ and Thy1.1 antibodies to quantitate percentage of clonotypic cells. Results are representative of 3 experiments.