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. 2007 Oct 11;117(11):3463–3474. doi: 10.1172/JCI31785

Figure 2. Decreased mitochondrial gene expression and function in MKOs.

Figure 2

(A) RNA was isolated from gastrocnemius muscle, and relative gene expression was measured by semiquantitative real-time PCR and normalized to 18S rRNA levels. NRF-1, nuclear respiratory factor 1; TFAM, mitochondrial transcription factor A; Ndufb5, NADH-ubiquinone oxidoreductase 1β subcomplex 5; Ndufs1, NADH-ubiquinone oxidoreductase Fe-S protein 1; Ndufv2, NADH-ubiquinone oxidoreductase flavoprotein 2; Cycs, cytochrome c; Atp5o, ATP synthase o subunit; ALAS-1, 5-aminolevulinate synthase. (B) Cross-sections of gastrocnemius were stained for Cox activity. (C) Cross-sections of gastrocnemius were stained for SDH activity. Scale bars: 250 μM. Values are mean ± SEM. *P < 0.05, MKO versus control. n = 8 per group.