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. Author manuscript; available in PMC: 2008 Sep 1.

Published in final edited form as: Immunity. 2007 Sep;27(3):438–452. doi: 10.1016/j.immuni.2007.07.017

Stromal cells are responsible for fatal multi-organ inflammation and increased numbers of effector/memory T cells in Bcl-3, NF-κB2 double knockout mice (dKO). (a) Bone marrow (BM) cells from NF-κB2 KO and dKO mice were transferred into lethally irradiated rag1 −/− mice. In addition, dKO bone marrow was depleted of CD4⁺ and CD8⁺ T cells prior to transfer into NF-κB2 KO OT-I mice, as shown. (b) Wild-type (WT) BM cells were transferred into lethally irradiated NF-κB2 OT-I and dKO OT-I mice. Chimeric mice were monitored and the surviving proportion was plotted (a, b). (c) Liver, lung and skin sections from BM chimeras were stained with H&E. Genotypes of BM donors and recipients were as indicated. (d) T cells from WT BM-reconstituted NF-κB2 KO OT-I and dKO OT-I mice were gated on CD4⁺ and analyzed for expression of CD44 and CD62L by flow cytometry. (e) T cells from WT BM-reconstituted dKO OT-I mice show elevated autoreactivity. APC = syngeneic antigen presenting cells. The graph shows increasing ratios of APC to constant amounts of CD4+ T cells. See Figure 2b for further details. Representative examples are shown and tissues and cells were from mice 6–7 weeks post transfer (c, d, e).

Stromal cells are responsible for fatal multi-organ inflammation and increased numbers of effector/memory T cells in Bcl-3, NF-κB2 double knockout mice (dKO). (a) Bone marrow (BM) cells from NF-κB2 KO and dKO mice were transferred into lethally irradiated rag1 −/− mice. In addition, dKO bone marrow was depleted of CD4⁺ and CD8⁺ T cells prior to transfer into NF-κB2 KO OT-I mice, as shown. (b) Wild-type (WT) BM cells were transferred into lethally irradiated NF-κB2 OT-I and dKO OT-I mice. Chimeric mice were monitored and the surviving proportion was plotted (a, b). (c) Liver, lung and skin sections from BM chimeras were stained with H&E. Genotypes of BM donors and recipients were as indicated. (d) T cells from WT BM-reconstituted NF-κB2 KO OT-I and dKO OT-I mice were gated on CD4⁺ and analyzed for expression of CD44 and CD62L by flow cytometry. (e) T cells from WT BM-reconstituted dKO OT-I mice show elevated autoreactivity. APC = syngeneic antigen presenting cells. The graph shows increasing ratios of APC to constant amounts of CD4+ T cells. See Figure 2b for further details. Representative examples are shown and tissues and cells were from mice 6–7 weeks post transfer (c, d, e).