Table 2.
IDC were cultured with and without METH (100 μM) for 24 hr (n = 3 independent experiments). Protein was extracted and subjected to DIGE as described in methods section. Data represent statistically significant differentially expressed proteins (Student’s t-test) that were identified using HPLC-MS/MS. Data are represented as protein name, gene accession number (Gi No.), average ratio, significance, % sequence coverage, theoretical mass, theoretical isoelectric point (pI), and molecular function (http://au.expasy.org/sprot/).
| Spot# | Protein Name | Accession (Gi No.) | Average ratio | P value | % sequence coverage | Theoretical Mass | Theoretical pI | Molecular Function |
|---|---|---|---|---|---|---|---|---|
| 130 | filamin 1 | gi|4503745| | 1.46 | 0.02 | 4.7 | 280761.06 | 5.73 | actin-binding protein |
| 130 | talin 1 | gi|14916725| | 1.46 | 0.02 | 2.4 | 296717.94 | 5.72 | actin-binding protein |
| 627 | CXCR3 | gi|20984919| | 1.76 | 0.001 | 28 | 88851.26 | 5.14 | chemokine receptor |
| 931 | Hsp70, protein 8 isoform 1 (HSPA8) | gi|5729877| | 1.70 | 0.04 | 5.4 | 70898.09 | 5.37 | molecular chaperone |
| 1129 | protein disulfide isomerase (PDI) | gi|7437388| | 1.53 | 0.014 | 30 | 56796.11 | 5.98 | protein disulfide reduction |
| 1426 | enolase | gi|4503571| | 1.38 | 0.01 | 43 | 47168.96 | 7.01 | glycolysis |
| 1668 | thioredoxin | gi|14249134| | 1.33 | 0.001 | 4.7 | 31361.01 | 4.93 | protein disulfide reduction |
| 1774 | unidentified | 1.64 | 0.05 | |||||
| 1787 | nuclear ribonucleoprotein A2/B1 | gi|7949053| | 1.25 | 0.01 | 22 | 35992.98 | 8.67 | Ribonucleoprotein nucleotide binding |
| 1787 | aldolase A | gi|229674| | 1.25 | 0.01 | 6.41 | 39288.83 | 8.39 | glycolysis |
| 1808 | cathepsin X | gi|7546545| | 1.33 | 0.05 | 36 | 27149.11 | 5.48 | cysteine-type peptidase activity |
| 1808 | inorganic pyrophosphatase | gi|11056044| | 1.33 | 0.05 | 20 | 32660.04 | 5.54 | hydrolase activity |
| 1951 | procathepsin B | gi|2982152| | 1.28 | 0.03 | 15 | 35167.57 | 5.89 | protease |
| 2317 | galectin 1 | gi|4504981| | 1.45 | 11 | 14715.70 | 5.33 | beta-galactoside-binding protein | |
| 1373 | coronin actin binding protein 1A | gi|5902134| | −1.49 | 0.04 | 5.9 | 51026.25 | 6.25 | actin binding |
| 1445 | pyruvate dehydrogenase alpha 1 | gi|4505685| | −1.63 | 0.04 | 6.1 | 43295.63 | 8.35 | oxidoreductase activity |
| 1639 | pyruvate kinase | gi|4505839| | −1.74 | 0.02 | 23 | 57913.85 | 7.95 | glycolysis |
| 1750 | calreticulin | gi|4757900| | −1.36 | 0.03 | 39 | 48141.56 | 4.29 | Ca(2+)-binding protein |
| 1930 | unidentified | −1.77 | 0.05 | |||||
| 2004 | tyrosine 3/tryptophan 5 - monooxygenase activation protein, zeta polypeptide | gi|4507953| | −1.78 | 0.004 | 31 | 27745.10 | 4.73 | signal transduction |
| 2012 | unidentified | −2.47 | 0.001 | |||||
| 2069 | dnaK-type molecular chaperone (HSPA5) | gi|87528| | −1.63 | 0.03 | 13 | 72115.65 | 5.03 | molecular chaperone |
| 2141 | peroxiredoxin | gi|2507169| | −1.42 | 0.03 | 29 | 21891.92 | 5.66 | antioxidant activity |