Table 3.
METH modulation of gene expression. IDC were treated with 100 μM METH for 24 hr. Relative mRNA species expression was quantitated and expressed as transcript accumulation index (TAI), calculated using the comparative CT method.
| Up-regulated Genes | ||
|---|---|---|
| Gene name | TAI | p value |
| filamin 1 | 1.52 ± 0.13 | 0.002 |
| talin 1 | 1.72 ± 0.23 | 0.001 |
| CXCR3 | 1.71 ± 0.24 | 0.006 |
| HSPA8 | 1.57 ± 0.23 | 0.015 |
| PDI | 1.32 ± 0.06 | 0.013 |
| Enolase | 1.49 ± 0.37 | 0.084 (NS) |
| Thioredoxin | 1.49 ± 0.05 | 0.001 |
| nuclear ribonucleoprotein A2/B1 | 1.45 ± 0.28 | 0.018 |
| aldolase A | 1.30 ± 0.12 | 0.029 * |
| cathepsin X | 1.60 ± 0.24 | 0.029 * |
| inorganic pyrophosphatase | 1.47 ± 0.18 | 0.011 |
| cathepsin B | 1.60 ± 0.39 | 0.029 * |
| galectin-1 | 1.39 ± 0.21 | 0.010 |
| Down-regulated Genes | ||
| Gene name | TAI | p value |
| Calreticulin | 0.42 ± 0.21 | 0.029 * |
| pyruvate dehydrogenase | 0.56 ± 0.19 | 0.004 |
| pyruvate kinase | 0.54 ± 0.22 | 0.007 |
| coronin actin binding protein 1A | 0.48 ± 0.09 | 0.029 * |
| tyrosine 3/tryptophan 5 - monooxygenase activation protein, zeta polypeptide | 0.53 ± 0.16 | 0.029 * |
| HSPA5 | 0.46 ± 0.11 | 0.001 |
| peroxiredoxin | 0.66 ± 0.08 | 0.002 |
Statistical significance was calculated by Student’s t-test or Mann-Whitney Rank Sum Test* (n = 3–4).