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. Author manuscript; available in PMC: 2008 Jul 15.
Published in final edited form as: Exp Cell Res. 2007 May 8;313(12):2575–2585. doi: 10.1016/j.yexcr.2007.04.030

Figure 2. Palladin is tyrosine phosphorylated in cells expressing active Src kinase.

Figure 2

HEK293 cells were co-transfected with HA-tagged palladin together with a constitutively active (CA) or a dominant-negative (DN) Src construct. Palladin was immunoprecipitated with anti-HA antibody and tyrosine phosphorylation analyzed with anti phosphotyrosine (pY) antibody. In cells expressing active Src, palladin is phosphorylated on tyrosine, whereas in cells expressing Src DN no phosphorylation is detected. As a negative control an HA-tagged amino-terminal (amino-acids 1–309/Y145F) ezrin construct was used.