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. Author manuscript; available in PMC: 2008 Jul 15.
Published in final edited form as: Exp Cell Res. 2007 May 8;313(12):2575–2585. doi: 10.1016/j.yexcr.2007.04.030

Figure 5. SPIN90 and palladin co-localize in cells expressing active Src.

Figure 5

U251 cells were co-transfected with a constitutively active (Src CA) or a dominant negative (Src DN) Src construct together with GFP-SPIN90 and stained for endogenous palladin with palladin Ab-3Ig antibody. Under basal conditions SPIN90 has a predominantly diffuse localization in the cytoplasm whereas palladin localizes to stress fiber dense bodies (Control). In the presence of Src DN, the localization of palladin and SPIN90 is similar to control cells. Expression of Src CA results in formation of numerous membrane projections and ruffles, which contain both palladin and SPIN90 (arrows).