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. Author manuscript; available in PMC: 2007 Oct 4.

Published in final edited form as: FEBS Lett. 2007 Jul 19;581(20):3809–3813. doi: 10.1016/j.febslet.2007.07.003

Insertion of truncated Aβ (stop) constructs into microsomal membranes. (A) The truncated Lep construct used. In addition to the G1 glycosylation site, a second Asn-X-Thr site (G2’) has been introduced just upstream of the Aβ (stop) segment (see Table S1). Constructs in which the Aβ (stop) segment is released from the membrane become glycosylated on both the G1 and G2’ sites (right), whereas constructs in which the Aβ (stop) segment is retained in the membrane are glycosylated only on the G1 site (left; see text). (B) In vitro translation in the presence of dog pancreas rough microsomes of constructs containing two different Aβ (stop) segments. Unglycosylated, singly glycosylated and doubly glycosylated forms of the protein are indicated by one open circle, one filled circle and two filled circles, respectively. The percentage of molecules not retained in the membrane is given below the lanes (average and standard deviation of at least three independent experiments).

Insertion of truncated Aβ (stop) constructs into microsomal membranes. (A) The truncated Lep construct used. In addition to the G1 glycosylation site, a second Asn-X-Thr site (G2’) has been introduced just upstream of the Aβ (stop) segment (see Table S1). Constructs in which the Aβ (stop) segment is released from the membrane become glycosylated on both the G1 and G2’ sites (right), whereas constructs in which the Aβ (stop) segment is retained in the membrane are glycosylated only on the G1 site (left; see text). (B) In vitro translation in the presence of dog pancreas rough microsomes of constructs containing two different Aβ (stop) segments. Unglycosylated, singly glycosylated and doubly glycosylated forms of the protein are indicated by one open circle, one filled circle and two filled circles, respectively. The percentage of molecules not retained in the membrane is given below the lanes (average and standard deviation of at least three independent experiments).