Fig. 5.

Short application Cl-IB-MECA did not modify fEPSP depression induced by 2-min OGD, but it significantly delays fEPSP recovery after 5-min OGD.

(A) Time course of fEPSP amplitude changes elicited by 2-min OGD in the absence and in the presence of Cl-IB-MECA (10 nM, n = 7). Data (mean ± S.E.M.) are expressed as a percentage of respective baseline values recorded before the respective OGD periods. As indicated by the open bar, the A₃ agonist was applied for only 2 min, during OGD. Note that short application of Cl-IB-MECA did not induce significant change (P > 0.05, paired two-tailed Student's t test) in the fEPSP depression induced by 2-min OGD. (B) Time course of fEPSP amplitude changes elicited by 5-min OGD in the absence (n = 20) and in the presence of Cl-IB-MECA (10 nM, n = 10). Data (mean ± S.E.M.) are expressed as a percentage of respective baseline values recorded before the respective OGD periods. As indicated by the open bar, the A₃ agonist was applied only for 2 min, at the end of 5-min OGD episodes. Note that short application of Cl-IB-MECA delayed fEPSP recovery after 5 min OGD. (C) Columns in the graph summarize the average time of fEPSP amplitude recovery (mean ± S.E.M.) after 5-min OGD in untreated OGD slices and during long or short application of Cl-IB-MECA. *P < 0.05, one-way ANOVA followed by Newman-Keuls multiple-comparison post hoc test versus untreated OGD slices; § P < 0.05, one-way ANOVA followed by Newman-Keuls multiple-comparison post hoc test versus all experimental groups. Solid bars indicate the duration of OGD.