The mRNA level of MTUS1 in HNSCC.
The qRT-PCR for MTUS1 was performed as described in Methods and Materials. The data analysis was carried out using the 2-delta delta Ct method described previously [22], where beta-actin was used as reference gene. A) The mRNA levels of MTUS1 were evaluated on 10 HNSCC cell lines and normal oral keratinocytes (NHOK). B) The mRNA levels of MTUS1 were evaluated on 10 pairs of tongue SCC cases and matching normal tissue samples.
The mRNA level of MTSU1 in HNSCC. Quantitative reverse transcriptase–polymerase chain reaction for MTUS1 was performed as described in Methods and Materials. Data analysis was performed using the 2–ΔΔCt method as described previously [22], with the β-actin gene ACTB used as the reference. (A) The mRNA levels of MTUS1 were evaluated on 10 HNSCC cell lines and normal oral keratinocytes (NHOK). (B) The mRNA levels of MTUS1 were evaluated on 10 pairs of oral tongue squamous cell carcinoma cases (OSCC) and matching normal tissue samples.