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Expression and purification of full-length Xenopus IRBP expressed in E. coli as a soluble thioredoxin/histidine-patch fusion protein (arrow). Coomassie blue stained 8% polyacrylamide gels, showing over expression of the recombinant IRBP. m = molecular weight markers; i = insoluble fraction; s = soluble fraction; "Pre" and "Post" refer to the bacterial fractions before and after induction with IPTG; p = purified protein.
