Table 2.
Summary of validation by WB and immunolocalization
| Protein | IPI | Function | ASAP Ratio | WB of mitochondria | WB of cytosol | Co-localized with cyt C* |
| MAP2K2 | 00135708 00407256 00231331 |
Signaling | 0 | Low sensitivity | Low sensitivity | N/A |
| Sacsin | 00124120 00373012 |
Folding degradation stability | 2.26 ± 0.38 | 1.77 ± 0.09 | 0.82 ± 0.02 | N/A |
| SNX1 | 00198369 00125441 |
Transport | 2.21 ± 0.6 | 1.51 ± 0.03 | Not detected | Yes |
| SHH | 00123314 | Signaling | 5.47 ± 0.98 | Low specificity | Low specificity | Yes |
| VAMP3 | 00132276 (00210971) |
Transport | 0.34 ± 0.07 | 0.48 ± 0.13 | 1.16 ± 0.15 | N/A |
*: Among the five candidate proteins, only antibodies against SHH and SNX1 demonstrated enough sensitivity and specificity for immunocytochemistry.