Table 3.
Primers, PCR conditions and qPCR efficiency
| Gene symbol | Oligo sequence (5'→3') | Amplicon length | Tm (°C) | E% |
| ACTB | CACGCCATCCTGCGTCTGGA AGCACCGTGTTGGCGTAGAG | 100 | 63 | 97 |
| B2M1 | CAAGATAGTTAAGTGGGATCGAGAC TGGTAACATCAATACGATTTCTGA | 161 | 58 | 101 |
| GAPDH | ACACTCACTCTTCTACCTTTG CAAATTCATTGTCGTACCAG | 90 | 60 | 103 |
| HMBS2 | AGGATGGGCAACTCTACCTG GATGGTGGCCTGCATAGTCT | 83 | 58 | 98 |
| HPRT1 | GGACTTGAATCATGTTTGTG CAGATGTTTCCAAACTCAAC | 91 | 60 | 99 |
| RPL4 | CAAGAGTAACTACAACCTTC GAACTCTACGATGAATCTTC | 122 | 60 | 97 |
| SDHA | CTACAAGGGGCAGGTTCTGA AAGACAACGAGGTCCAGGAG | 141 | 58 | 98 |
| TBP1 | AACAGTTCAGTAGTTATGAGCCAGA AGATGTTCTCAAACGCTTCG | 153 | 60 | 93 |
| YWHAZ | TGATGATAAGAAAGGGATTGTGG GTTCAGCAATGGCTTCATCA | 203 | 60 | 99 |