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. 2007 Jul 30;7:91. doi: 10.1186/1471-213X-7-91

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Copyright © 2007 Chapman et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Streak to RBI quail-chick chimera transplants and Wnt8c expression. ISH (Wnt8c, blue) and immunochemistry (anti-QCPN antibody, brown). Anterior to the top, (F) Level of 50 μm gelatin section marked by black arrows. (A) Schematic of experiment with donor quail tissue from rostral or caudal streak transplanted to chick rostral blastoderm isolate (RBI) and analyzed using Wnt8c ISH marker. Whole mount inset shows Wnt8c is restricted to the caudal two-thirds of the primitive streak and ingressed mesoderm directly adjacent to the primitive streak. (B, C) As expected, Wnt8c is not expressed in transplanted rostral streak or RBI at either 4–6 hours or overnight incubation. (D) Wnt8c expression is down regulated in caudal cells transplanted to RBIs after 4–6 hours. (E) Caudal streak transplanted to the RBI is unable to express Wnt8c after overnight incubation, but the explant induces Wnt8c in the chick host tissue (F) Section with Wnt8c expression in chick tissue and negative for expression in quail cells.