Table 2.
Changes observed in the post-translational modification of proteins as a result of bldA mutation
| Gene | Function1 | Alteration in protein abundance2 observed for: | |
| Spot 1 | Spot 2 | ||
| A) Spots differ in isoelectric point (pI): spot 1 has more acidic pI than spot 2 | |||
| SCO1246 | BioD (dethiobiotin synthetase) | >2-fold up in ΔbldA | >2-fold down in ΔbldA |
| SCO1916 | Putative transferase | Absent in ΔbldA | No change |
| SCO2390 | FabF (3-oxoacyl- [ACP] synthase II | No change | >2-fold down in ΔbldA |
| SCO2618 | ClpP2 (Clp-protease subunit 2) | >2-fold up in ΔbldA | >2-fold down in ΔbldA |
| SCO3073 | HutU (urocanate hydratase) | No change | Absent in ΔbldA |
| SCO3137 | GalE1 (UDP-glucose 4-epimerase) | Absent in ΔbldA | No change |
| SCO3409 | Ppa (inorganic pyrophosphatase) | >2-fold up in ΔbldA | >2-fold down in ΔbldA |
| SCO4164 | CysA (thiosulphate supfurtransferase) | >2-fold up in ΔbldA | >2-fold down in ΔbldA |
| SCO7400 | ABC transport protein | >2-fold down in ΔbldA | No change |
| B) Spots differ in Mwt (and pI): spot 1 has higher Mwt than spot 2 | |||
| SCO2271 | Putative membrane protein | >2-fold down in ΔbldA | No change |
| SCO5465 | Conserved hypothetical protein | Absent in ΔbldA | >2-fold up in ΔbldA |
1from ScoDB [55]
2protein abundance profiles for all 22 protein spots are detailed in Additional File 7.