Table 4.
Genes that have a high confidence in congruence between protein and transcript profile during growth
Gene/spot | Confidence ((1-p) × 100) | Spearman rank correlation | Annotated gene function |
Positive correlation in set A | |||
SCO0379:123 | 100 | 1 | catalase KatA |
SCO1651 | 100 | 1 | conserved hypothetical protein |
SCO1776 | 100 | 1 | putative CTP synthetase PyrG |
SCO2554 | 100 | 1 | DnaJ |
SCO7510 | 100 | 1 | peptidyl-prolyl cis-trans isomerase CypH |
Positive correlation in set B | |||
SCO1212 | 100 | 1 | putative ligase |
SCO2115 | 100 | 1 | putative 2-dehydro-3-deoxyphosphoheptonate aldolase AroH |
SCO2539 | 100 | 1 | Era-like GTP-binding protein |
SCO3801 | 100 | 1 | putative aminopeptidase |
SCO3907 | 100 | 1 | single strand DNA-binding protein ssb |
SCO4809 | 100 | 1 | succinyl CoA synthetase alpha chain SucD |
SCO4813 | 97.7 | 0.87 | phosphoribosylglycinamide formyltransferase PurN |
SCO4824 | 98.4 | 0.89 | bifunctional protein (methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase FolD |
SCO4921 | 100 | 1 | putative acyl-CoA carboxylase complex A subunit AccA2 |
SCO5745 | 100 | 1 | conserved hypothetical protein |
Positive correlation in set C | |||
SCO0379:123 | 97 | 0.85 | catalase KatA |
SCO1244 | 98 | 0.88 | Biotin synthase BioB |
SCO1489 | 98.4 | 0.89 | DNA-binding protein BldD |
SCO1523 | 98.4 | 0.92 | conserved hypothetical protein |
SCO1651 | 98.4 | 0.89 | conserved hypothetical protein |
SCO1776 | 99.8 | 0.96 | putative CTP synthetase PyrG |
SCO2949 | 98 | 0.88 | UDP-N-acetylglucosamine transferase MurA |
SCO3122 | 98.4 | 0.9 | putative nucleotidyltransferase |
SCO4550 | 98.4 | 0.9 | conserved hypothetical protein |
SCO4813 | 97.7 | 0.87 | phosphoribosylglycinamide formyltransferase PurN |
SCO4824 | 98.4 | 0.89 | bifunctional protein (methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase FolD |
SCO5113 | 98.4 | 0.9 | BldKB, putative ABC transport system lipoprotein, |
Negative correlation (all in set C) | |||
SCO1947:683 | 98.4 | -0.86 | glyceraldehyde-3-phosphate dehydrogenase Gap1 |
SCO2198:235 | 97.7 | -0.87 | glutamine synthetase I GlnA |
SCO2198:746 | 98.8 | -0.92 | glutamine synthetase I GlnA |
SCO2634 | 97 | -0.85 | conserved hypothetical protein |
SCO2736:472 | 95.1 | -0.83 | Citrate synthase CitA |
SCO5466 | 97.8 | -0.87 | putative hydrolase |
Congruence across time was calculated by Spearman Rank Correlation given different data sets where set A represents 5 time points in M600, B represents 5 time points in ΔbldA and C represents 10 time points spanning both M600 and ΔbldA (the 5 time points are 1, 2, 4, 6 and 8 in Fig. 1).