Table 2.
Yeast-2-Hybrid analysis of Ppa-MBD-2
| Gene | -LTH+3AT1 | LacZ |
| mbd-2 | - | NA |
| dlc2,3 | + | + |
| T07C4.102,3 | + | + |
| bath-382,3 | + | + |
| tag-2602,3 | + | + |
| hip-12,3 | + | + |
| 2 × No sim.2,3 | + | + |
1 The assay was performed on plates depleted in leucine, triptophane and histidine, supplemented with 50 mM 3 AT. 2 Results were confirmed by shifting prey and bait constructs. mbd-2, autoactivation control (pDEST-32-Ppa-mbd-2). 3 Interactors found in the screen of the cDNA library. dlc, dineyn light chain. T07C4.10, myosin heavy chain homolog. Bath-38, BTB/MATH/POZ domain similar to Spop. Tag-260, proteins similar to C. elegans tag-260, annotated as a predicted E3-ubiquitin ligase. Hip-1, Huntingtin interacting protein, 2 × No sim, 2 clones with no homolog in the database. NA, not-applicable.