Figure 1.
CD8α is detected by flow cytometry on CD14hi monocytes from human peripheral blood. Anti-CD8α mAb bind monocytes by flow cytometry. A, Monocytes were gated by characteristic FSC/SSC scatter (left panel) and expression of high levels of CD14 (right panel) for all flow cytometry studies in this article. B, A population of lymphocytes, gated by characteristic FSC/SSC scatter, bind high levels of anti-CD8α. Blood monocytes gated in A, bind anti-CD8α mAb. Geometric means of monoclonal antibody binding are shown. Results are representative of five experiments. C, Anti-CD8β mAb bind lymphocytes but not monocytes.