Figure 5.
CD8α on human peripheral blood monocytes mediates MHC class I binding. A, Dark histogram is background fluorescence of CD14hi monocytes, light histogram represents binding of PE-labeled tetramers. B, Bar graph is pooled results of gated CD14hi monocytes from three different donors in separate experiments. Inhibition of tetramer binding is expressed as percent decrease in mean fluorescence intensity of tetramer binding due to pretreatment with anti-CD8α mAb compared to isotype mAb (* p < 0.05, non-paired t-test).