Figure 7.
CD8α enhances monocyte TNF production in a FcγR-dependent manner. Monocytes were incubated with anti-CD8α or isotype mAb alone or in immune-complexes. Excess Fc fragment was used to block FcγR. TNF production was measured by intracellular flow cytometry after 5 h (gated on CD14hi monocytes). A. Histograms are representative results. B. Bar graph represents average geometric mean of intracellular TNF from four separate experiments with monocytes from different donors. Standard error of the mean is designated by error bars. *: p < 0.05 non-paired t-test. C. CD69 and CD14 regulation induced by immune-complexes (IC) is enhanced by CD8α ligation (representative example).