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. 2007 Oct 5;3(10):e141. doi: 10.1371/journal.ppat.0030141

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Eight mice were inoculated with recombinant wt 1918 virus at 10², 10³, 10⁴, 10⁵, or 10⁶ PFU, and their weights were measured every day. Average weights are represented in the graph.

(B) Eight mice were inoculated with recombinant 1918 S66N virus at 10²,10³, 10⁴, 10⁵, or 10⁶ PFU, and their weights were measured every other day. Average weights are represented in the graph.

(C) Effect of S66N mutation on mouse lung titers in r1918 virus. Virus titers from lung homogenates were measured from 3 mice infected per time point with r1918 or r1918 S66N virus at days 1, 2, 3, 6, 7, and 8 after inoculation. Error bars represent 1 standard deviation.