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. 2007 Oct 5;3(10):e142. doi: 10.1371/journal.ppat.0030142

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© 2007 Manayani et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Ribbon diagram of a single FHV coat protein subunit showing the surface-exposed loops into which the VWA domain of ANTXR2 was inserted.

(B) Schematic diagram showing structure of FHV-VWA_ANTXR2 chimeric proteins 206 (top) and 264 (bottom). Numbers refer to amino acid positions in the FHV coat protein. The assembly-dependent autocatalytic maturation cleavage occurs between Asn 363 and Ala 364.

(C) Electrophoretic analysis of purified VLPs on a 10% Bis-Tris gel stained with Simply Blue (Invitrogen). Lane 1, molecular weight markers; lane 2, wt FHV VLPs; lane 3, FHV-VWA_ANTXR2 chimera 206; lane 4, FHV-VWA_ANTXR2 chimera 264.

(D) Electron micrographs of gradient-purified wt and chimeric VLPs negatively stained with uranyl acetate. Bar = 500 Å.