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. Author manuscript; available in PMC: 2007 Oct 5.
Published in final edited form as: Arch Biochem Biophys. 2006 Aug 22;454(1):42–54. doi: 10.1016/j.abb.2006.08.003

Figure 2.

Figure 2

Purification and identification of the C98S mutant tryptic peptide adduct photolabeled with lapachenole. HPLC separation of the proteolytic digests of the lapachenole labeled CYP3A4 monitored by (a) variable wavelength detection at 214 nm and (b) fluorescence detection using 320 nm and 420 nm as excitation and emission wavelengths, respectively. The fraction containing one major fluorescence peak F-1 eluted at 27.2 min was collected and analyzed by nano-LC/ESI MS.