Table 2.

Primers and PCR programs for CTLA-4 PCR-RFLP genotyping

Reference SNP ID	Gene/SNP primer	Sequence	PCR program
rs4553808	CTLA-4 -1661G/A F	5'-CTAAGAGCATCCGCTTGCACCT-3'	94°C 5 min; 30 cycles, 94°C 15s,
	CTLA-4 -1661G/A R	5'-TTGGTGTGATGCACAGAAGCCTTT-3'	58°C 5s, 72°C 45s; 72°C 7 min
rs11571317	CTLA-4 -658T/C F	5'-ATCATTGGTCCTGTCTACAGC-3'	94°C 5 min; 35 cycles, 94°C 30s,
	CTLA-4 -658T/C R	5'-CTTCTAATGGTCCCTTGACAG-3'	55.5°C 10s, 72°C 30s; 72°C 5 min
rs5742909	CTLA-4 -318T/C F	5'-AAATGAATTGGACTGGATGGT-3'	94°C 4 min; 30 cycles, 94 °C 30s,
	CTLA-4 -318T/C R	5'-TTACGAGAAAGGAAGCCGTG-3'	55.6°C 5s, 72°C 30s; 72°C 4 min
rs231775	CTLA-4 +49G/A F	5'-AGTCTCACTCACCTTTGCAG-3'	94°C 4 min; 30 cycles, 94°C 30s,
	CTLA-4 +49G/A R	5'-GCTCTACCTCTTGAAGACCT-3'	56.4°C 10s, 72°C 30s; 72°C 4 min
rs3087243	CTLA-4 CT60G/A F	5'-GAGGTGAAGAACCTGTGTGTTAAA-3'	94°C 5 min; 30 cycles, 94°C 30s,
	CTLA-4 CT60G/A R	5'-ATAATGCTTCATGAGTCAGCTT-3'	55.6°C 30s, 72°C 30s; 72°C 5 min