Table 2.

The difference in characteristics of 5'-end proximal hairpins started in 6^th – 20^th CDS positions of human and mouse cDNAs characterized by optimal and suboptimal start codon contexts (purine (Pu) and pyrimidine (Py) in pos. -3 upstream AUG, respectively; E_tot, energy of an eligible hairpin, kcal/mol; E_st, energy of the hairpin stem region, kcal/mol; L_st, size of the hairpin stem region, base pairs)†

CDS pos.	human			mouse
	EtotPy-EtotPukcal/mol	EstPy-EstPu kcal/mol	LstPy-LstPu b.p.	EtotPy-EtotPu kcal/mol	EstPy-EstPu kcal/mol	LstPy-LstPu b.p.
6	0.45	0.21	-0.14	0.09	-0.16	0.08
7	0.40	0.38	-0.19	0.02	0.35	-0.26
8	-0.25	0.15	-0.22	1.04	-0.027	-0.16
9	1.79	0.11	-0.203	0.24	-0.53	0.02
10	1.89	0.01	0.13	-0.47	-0.29	0.11
11	0.79	0.21	-0.09	-0.87	-0.80	0.16
12	1.12	0.11	-0.02	0.86	1.29	-0.64
13	-1.28*	0.39	-0.13	-3.65*	-1.02*	0.19
14	-2.41*	-1.09**	0.10	1.12	1.65	-0.74
15	-0.87	-0.29	0.09	-1.84	0.54	-0.47
16	-1.03**	-0.53*	0.26*	3.42	-0.10	0.11
17	2.87	-1.15**	0.71*	-3.73*	-0.60*	0.46
18	-0.94	0.13	-0.24	3.08	-0.43	0.04
19	-2.99*	0.82	-0.48	1.88	0.75	0.10
20	-1.82	-0.58	0.06	-2.74	0.47	-0.32

† CDS positions where mean E_tot and E_st values were significantly lower (and L_st values were significantly higher) in mRNA samples with a suboptimal start codon context are marked (E_tot^Py – E_tot^Pu, E_st^Py – E_st^Pu, and L_st^Py – L_st^Pu values are bolded; the significance level (according to Mann-Whitney U-test or Kolmogorov-Smirnov two-sample test) is shown by numbers: * p < 0.05; ** p < 0.01;*** p < 0.001)