(A) Stern-Volmer plots of F0/F versus [acrylamide] for single-stranded (SS, diamonds), double-stranded (DS, squares) and A-site (AS, circles) RNAs. (B) Stern-Volmer plots for SS and DS in the absence (closed symbols) and presence (open symbols) of 10 μM paromomycin. All experiments were carried out in triplicate. The Ksv values, determined by using Equation 1 (experimental section), are given in Table 1.