The LLnL-induced MHC class I heavy chain derivative is not glycosylated. Daudi (A) and .174 (B) cells were treated with 250 μM LLnL or DMSO solvent control for 2 hr at 37°C, separated into membrane and soluble fractions, immunoprecipitated using the mouse antibody HC10, treated with endo H or N-glyF, subjected to SDS/PAGE, transferred to Immobilin P membrane, and visualized using the rat antibody 3B10.7. Solid arrowheads indicate glycosylated class I heavy chain. Shaded arrowheads indicate deglycosylated class I heavy chain.