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. 1997 Mar 4;94(5):1896–1901. doi: 10.1073/pnas.94.5.1896

Figure 4.

Figure 4

The LLnL-induced MHC class I heavy chain derivative is located in the cytosol. Daudi and .174 cells were treated with 250 μM LLnL or DMSO solvent control for 4 hr at 37°C. The cells were treated with SLO for 30 min at 20°C and separated into lumenal (L) or cytosolic (C) fractions by centrifugation. Class I heavy chains were immunoprecipitated using HC10, subjected to SDS/PAGE, and visualized using 3B10.7. The soluble, ER-retained chaperone, grp94 (gp96), was immunoprecipitated and visualized using the rat mAb clone 9G10. The soluble, cytosolic control molecule, Hsc70, was immunoprecipitated and visualized using the rat antibody 1B5.