Figure 5.

ICP47 induces the soluble MHC class I heavy chain derivative in 45.1. (A) The cells lines 45.1 and 45.1.ICP47 were analyzed for class I surface expression by flow cytometry using the mAb w6/32 and fluorescein-conjugated rabbit anti-mouse IgG. The control was a nonspecific isotype-matched mAb. (B) The lines 45.1 and 45.1.ICP47 were treated with 250 μM LLnL for 2 hr at 37°C and separated into membrane (M) and soluble (S) fractions by ultracentrifugation. MHC class I heavy chain was immunoprecipitated after addition of Triton X-100 using mouse antibody HC10, subjected to SDS/PAGE, transferred to Immobilon P membrane, and visualized using the rat antibody 3B10.7.