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Binding of scFv anti-F(ab′)2-expressing phages to F(ab′)2 fragments. (A) The polyclonal phage population obtained after each selection round was tested in ELISA for binding to F(ab′)2. Negative controls consisted of microtiter plates coated with blocking buffer or BSA. (B) Single clones (n = 95) with anti-F(ab′)2 activity were generated and tested in ELISA (negative controls: A620 of blocking buffer = 0.079 and that of BSA = 0.074).
