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Competitive inhibition of serum IgG-anti-F(ab′)2 antibodies with scFv anti-F(ab′)2 antibodies. ELISA plates coated with F(ab′)2 γ-fragments were incubated with increasing dilutions of scFv2 (A), scFv6 (B), and a human anti-digoxigenin scFv antibody (C). Serum IgG-anti-F(ab′)2 antibodies were added at a dilution of 1:512. Negative controls consisted of PBS. Maximum binding activity of scFv2 and scFv6 was A620 = 0.983 and 0.869, respectively.
