Table 1.
V gene usage of anti-F(ab′)2 antibodies
Clone | Closest germ-line V gene | Homology, % | Gene family | Closest rearranged V gene | Homology, % | Accession no. |
---|---|---|---|---|---|---|
HC 1,3-12 | DP-29 | 88.7 | VH3 | Ig H chain from a B cell tumor | 89.2 | Z31394Z31394 |
HC 2 | DP-29 | 88.3 | VH3 | Ig H chain from a B cell tumor | 89.4 | Z31394Z31394 |
LC 1,3-12 | DPK-15 | 100 | Vκ2 | Ig κ L chain from a spleen library | 99.2 | X72452X72452 |
LC 2 | DPK-15 | 99.3 | Vκ2 | Ig κ L chain from a spleen library | 97.5 | X72452X72452 |
Comparison of the anti-F(ab′)2 scFv antibodies’ variable heavy (H) and light (L) chains with the V BASE (Medical Research Council, Cambridge, U.K. CB2 2QH) the GenBank and EMBL databases.