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. 1997 Mar 4;94(5):1902–1907. doi: 10.1073/pnas.94.5.1902

Table 2.

Kinetic parameters for binding of scFv fragments to F(ab′)2 measured by the BIAcore system

Antibody fragment kon, M−1·s−1/104 koff, s−1/10−4 Ka = kon/koff, M−1/107 Keq, M−1/107
scFv2 2.47  ±  0.11 8.86  ±  0.24 2.78  ±  0.80 2.29  ±  0.63
scFv6 0.74  ±  0.09 9.26  ±  0.81 0.79  ±  0.07 0.60  ±  0.18

Association and dissociation rate constants were calculated using the homogeneous (monophasic) kinetic model. Affinity (equilibrium association) constants were obtained directly from the ratio kon/koff as well as from steady-state measurements (Keq).