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. 1997 Mar 4;94(5):1919–1924. doi: 10.1073/pnas.94.5.1919

Figure 1.

Figure 1

Structure and expression of porcine syk mutants. (A) Schematic representation of syk cDNA constructs used for construction of recombinant vaccinia viruses. SH2(N), NH2 terminal Src homology 2 domain; SH2(C), COOH terminal Src homology 2 domain; ID1, interdomain region between the tandem SH2 domains; ID2, interdomain region between SH2(C) and the tyrosine kinase domain. The amino acid residues of wild-type syk that are mutated in the syk-K (Lys-395 to arginine) and syk-AL mutants (Tyr-518 and Tyr-519 to phenylalanine) are indicated. (B) Expression of various syk mutants in RBL-2H3 cells. RBL-2H3 cells were infected with the indicated viruses for 14 h. Cells were then lysed and analyzed by immunoblotting with an anti-porcine syk-specific antibody.